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Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (12)

Interestingly, BAL fluid IL-8 and PLA were elevated in asymptomatic HIV-positive patients in comparison with that in normal volunteers, and these patients had abnormal Pa02 (86 ±6.7 mm Hg) and P(A-a)02 values (26.5 ±3.6). Other investigators have demonstrated increased production of tumor necrosis factor a by alveolar macrophages from HIV-positive patients without pulmonary infections. In…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (11)

That PLAg, but not LTB4, was significantly elevated in patients with P carinii pneumonia suggests that while LTB4 does not play a role in the lung injury of P carinii pneumonia, other products of PLAa action (eg, lysophospholipids and free fatty acids) may contribute to pulmonary dysfunction. Elevation of PLAa levels in P carinii pneumonia…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (10)

The BAL fluid IL-8 was elevated in HIV-infected patients with P carinii pneumonia and correlated with the BAL fluid absolute neutrophil count. These data suggest that IL-8 mediates recruitment and activation of neutrophils in P carinii pneumonia and may thereby contribute to the hypoxia seen in this infection. Interleukin-8 also may play a role in…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (9)

Discussion Despite recent growing interest in the biology of P carinii and pathophysiology of P carinii pneumonia, little is known of the pathogenesis of lung injury in this disease. Similarly, the pathogenesis of NIP, a frequent cause of respiratory symptoms in patients with HIV infection, remains obscure. Several groups have suggested that cytokines, particularly tumor…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (8)

Pneumocystis carinii Pneumonia Bronchoalveolar lavage fluid IL-8 was elevated in patients with P carinii pneumonia compared with that in normal volunteers (p = 0.009; Fig IB) and correlated with absolute neutrophil count (r=0.73; p = 0.005) but not with P(A-a)02 (r = 0.57; p = 0.11). BAL fluid LTB4 was not elevated in patients with…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (7)

Interleukins Interleukin-8 was measured in unconcentrated BAL using a commercially available enzyme-linked immunosorbent assay kit (Research and Diagnostic Systems, Minneapolis). The detection limit of this assay is 18 pg/ml. Statistical Analysis Values for BAL LTB4, IL-8, PLA, and absolute neutrophil count are reported as mean± SEM. Differences between groups were evaluated using the Wilcoxon rank…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (6)

Radioimmunoassay for Leukotriene B4 Leukotriene B4 was measured in HPLC fractions and extracts of BAL supernatants by radioimmunoassay (LTB4 antiserum, Advanced Magnetics, Cambridge, Mass). The detection limit of this assay is 8.2 picogram (pg)/sample.

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (5)

High Performance Liquid Chromatography High performance liquid chromatography was performed as previously described on a Beckman model 344 liquid chromatography system (Beckman Instruments Inc, Fullerton, Cal) using an Ultrasphere C18 column (4.7 x 250 mm) with 5 |un particle size (Beckman) together with an Ultrasphere C18 precolumn (4.7 x 45 mm). The gradient program of…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (4)

The remaining fluid was centrifuged at 4°C for 10 min at 1,500 g, and the clarified supernatant was saved at — 70°C until assayed. Prostaglandin Bt (200 ng; Sigma Chemical Co, St. Louis, Mo) was added to one stored aliquot from each patient to serve as an internal standard for future high performance liquid chromatography…

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (3)

Materials and Methods Collection of Clinical Specimens Bronchoalveolar lavage specimens were collected from patients undergoing diagnostic evaluation for suspected pulmonary disease, volunteers enrolled in a research protocol investigating HIV-positive patients without respiratory symptoms, and five normal volunteers. Only one patient of those from whom data were available (30 of 39 patients) was a smoker. No…

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