Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (7)

Interleukins
Interleukin-8 was measured in unconcentrated BAL using a commercially available enzyme-linked immunosorbent assay kit (Research and Diagnostic Systems, Minneapolis). The detection limit of this assay is 18 pg/ml.
Statistical Analysis
Values for BAL LTB4, IL-8, PLA, and absolute neutrophil count are reported as mean± SEM. Differences between groups were evaluated using the Wilcoxon rank sum test; the Bonferroni correction for multiple comparisons was used where applicable. Significance was defined as a probability value less than 0.05. Bronchoalveolar lavage measurements were correlated with alveolar-arterial oxygen pressure difference P(A-a)0, and absolute neutrophil count using linear regression.
Results
Patients
BAL specimens from 34 HIV-infected patients and five normal volunteers were analyzed. Nineteen patients had P carinii pneumonia, ten had NIP (five with pulmonary symptoms prompting bronchoscopy and five asymptomatic patients who underwent bronchoscopy under an investigative protocol), and five were asymptomatic patients enrolled in the protocol mentioned above who had no pathologic diagnosis made. None of these patients died within 1 month of bronchoscopy. Clinical and laboratory data are presented in Table 1.

Table 1—Clinical Characteristics and Bronchoalveolar Lavage Measurements of Batients and Control Subjects

Patient	Age,	Sex	RiO*, mm Hg	P(A-a)O_e, mm Hg	ltb₄,pgfail	IL-8,pg/ml	PLA*,disintegrations/min	Absolute Neutrophil Count, cells/ml
Normal volunteers
1	21	M	nm	nm	14	71	1,211	0
2	30	F	nm	nm	2	86	0	5,842
3	40	M	nm	nm	3	57	632	656
4	21	M	nm	nm	15	34	0	3,930
5	23	M	nm	nm	17	50	nm	0
Asymptomatic HIV-positive patients
6	40	M	87	22	4	nm	2,602	1,600
7	40	M	99% saturated!	nm	30	119	9,480	6,900
8	41	M	80	31	36	252	3,444	620
9	29	M	82	34	12	205	1,585	0
10	55	M	95	19	17	962	1,470	5,100
NIP, asymptomatic
11	36	M	84	22	33	nm	1,213	1,660
12	32	M	85	24	62	1,616	14,708	48,750
13	24	M	117	2	37	364	7,642	16,800
14	29	M	74	37	69	nm	nm	40,000
15	28	M	93	14	36	425	1,921	0
NIP, symptomatic
16 43		M	109	2	65	64	35,709	nm
17	28	M	68	48	122	2,216	23,064	360,000
18	37	M	78	38	24	1,317	4,694	800
19	43	M	88	27	22	nm	14,976	300
20	37	M	89	24	81	nm	nm	0
P carinii pneumonia
21	33	M	114	9	6	255	11,734	3,600
22	33	M	94	27	6	784	41,561	0
23	59	M	nm	nm	10	59	802	160
24	29	M	nm	nm	17	491	46,364	15,600
25	31	M	96% saturated!	nm	14	107	1,287	700
26	29	M	50	61	13	nm	615	nm
27	37	M	97	18	12	nm	nm	0
28	51	M	86	29	16	866	3,943	nm
29	34	M	96	22	32	69	695	0
30	40	M	104	16	12	647	23,375	nm
31	35	M	90	25	15	384	15,363	nm
32	55	M	79	nm	9	1,176	42,062	6,300
33	42	M	78	34	20	278	9,029	5,700
34	33	M	93% saturated!	nm	34	nm	53,539	3,240
35	25	M	98% saturated!	nm	32	141	3,989	0
36	45	M	nm	nm	15	309	4,936	0
37	29	M	74	38	23	2,700	3,296	264,000
38	28	M	93	14	6	543	15,696	5,600
39	39	M	nm	nm	9	485	1,176	19,200

Category: Pulmonary disease

Tags: absolute neutrophil counts, bronchoalveolar lavage, Bronchoscopy, carinii pneumonia, hiv-positive patients

Leukotriene B4 and lnterleukin-8 in Human Immunodeficiency Virus-related Pulmonary Disease (7)

Product List

Asthma Inhaler List

News